human prorenin enzyme Search Results


recombinant human prorenin  (Innovative Research Inc)
90
Innovative Research Inc recombinant human prorenin
Recombinant Human Prorenin, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hba1c elisa kit  (Shanghai Korain Biotech Co Ltd)
94
Shanghai Korain Biotech Co Ltd human hba1c elisa kit
Human Hba1c Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa duo set kits  (R&D Systems)
93
R&D Systems elisa duo set kits
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Elisa Duo Set Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits  (R&D Systems)
95
R&D Systems elisa kits
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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renin polyclonal igg b 12 antibody  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology renin polyclonal igg b 12 antibody
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Renin Polyclonal Igg B 12 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant prorenin  (Cayman Chemical)
90
Cayman Chemical human recombinant prorenin
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Human Recombinant Prorenin, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant rat prorenin  (Innovative Research Inc)
90
Innovative Research Inc recombinant rat prorenin
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Recombinant Rat Prorenin, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit  (Cusabio)
90
Cusabio elisa kit
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal anti human renin antibodies br1 512  (Inserm Transfert)
86
Inserm Transfert polyclonal anti human renin antibodies br1 512
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Polyclonal Anti Human Renin Antibodies Br1 512, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human prorenin  (Innovative Research Inc)
90
Innovative Research Inc human prorenin
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Human Prorenin, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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female double transgenic rats  (Bohlender gmbh)
90
Bohlender gmbh female double transgenic rats
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Female Double Transgenic Rats, supplied by Bohlender gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti renin h 105 antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology rabbit polyclonal anti renin h 105 antibody
Figure 1. PDWGF digest <t>induces</t> <t>IL-1b,</t> IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by <t>ELISA.</t> Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001
Rabbit Polyclonal Anti Renin H 105 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. PDWGF digest induces IL-1b, IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by ELISA. Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001

Journal: PloS one

Article Title: Pepsin digest of wheat gliadin fraction increases production of IL-1β via TLR4/MyD88/TRIF/MAPK/NF-κB signaling pathway and an NLRP3 inflammasome activation.

doi: 10.1371/journal.pone.0062426

Figure Lengend Snippet: Figure 1. PDWGF digest induces IL-1b, IL-18, and IL-1a release in monocytes and PBMC from CD patients. IL-1b (A), IL-18 (B) and IL-1a (C) levels were quantified in cell supernatants by ELISA. Data are given as mean 6 SD from 39 patients and 15 healthy donors (HD). *P,0.05, **P,0.01 (CD vs. HD). (D). PDWGF-induced activation of PBMC is not due to LPS contamination. Results are shown as the percentage of the cytokine production from 4 CD patients. The data were normalized to the result from untreated cells which was set as 100%. Mean 6 SD, n = 4 independent experiments, ***P,0.001 compared to untreated cells. doi:10.1371/journal.pone.0062426.g001

Article Snippet: The concentrations of human IL-1b, IL-1a, IL-18 and TNF-a as well as murine IL-1b and TNF-a were measured by commercial ELISA Duo Set Kits (R&D Systems) or ELISA MAX kits (Biolegend) according to manufacturer instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay

Figure 3. PDWGF-induced IL-1b production from celiac patient PBMC is modulated by K+ efflux, but is independent of the P2X7 receptor; as shown by (A) ELISA, mean 6 SD, n = 10, ***P,0.001 vs. PDWGF-treated cells; and by (B) Western blot. Representative blots from 5 independent experiments are shown. (C) Inhibition of ROS modulate PDWGF-induced IL-1b secretion, mean 6 SD, n = 10; as well as (D) pro-IL-1b production from PBMC of CD patients. Representative blots from 3 independent experiments are shown. b-actin was used as a loading control. ***P,0.001 vs. PDWGF- treated cells. doi:10.1371/journal.pone.0062426.g003

Journal: PloS one

Article Title: Pepsin digest of wheat gliadin fraction increases production of IL-1β via TLR4/MyD88/TRIF/MAPK/NF-κB signaling pathway and an NLRP3 inflammasome activation.

doi: 10.1371/journal.pone.0062426

Figure Lengend Snippet: Figure 3. PDWGF-induced IL-1b production from celiac patient PBMC is modulated by K+ efflux, but is independent of the P2X7 receptor; as shown by (A) ELISA, mean 6 SD, n = 10, ***P,0.001 vs. PDWGF-treated cells; and by (B) Western blot. Representative blots from 5 independent experiments are shown. (C) Inhibition of ROS modulate PDWGF-induced IL-1b secretion, mean 6 SD, n = 10; as well as (D) pro-IL-1b production from PBMC of CD patients. Representative blots from 3 independent experiments are shown. b-actin was used as a loading control. ***P,0.001 vs. PDWGF- treated cells. doi:10.1371/journal.pone.0062426.g003

Article Snippet: The concentrations of human IL-1b, IL-1a, IL-18 and TNF-a as well as murine IL-1b and TNF-a were measured by commercial ELISA Duo Set Kits (R&D Systems) or ELISA MAX kits (Biolegend) according to manufacturer instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Inhibition, Control

Figure 4. MAPK and NF-kB are involved in PDWGF mediated IL- 1b secretion. PDWGF-primed PBMC were treated with SB203580 (SB), SP600125 (SP), UO125 (UO), TPCK, or PDWGF, alone or in combination for 24 h. (A) IL-1b was quantified in cell supernatants by ELISA. Mean 6 SD, n = 5 independent experiments. ***P,0.001 vs. PDWGF-treated cells. (B) Pro-IL-1b levels were examined in cell lysates by immunoblot- ting from 5 experiments. b-actin was used as a loading control. doi:10.1371/journal.pone.0062426.g004

Journal: PloS one

Article Title: Pepsin digest of wheat gliadin fraction increases production of IL-1β via TLR4/MyD88/TRIF/MAPK/NF-κB signaling pathway and an NLRP3 inflammasome activation.

doi: 10.1371/journal.pone.0062426

Figure Lengend Snippet: Figure 4. MAPK and NF-kB are involved in PDWGF mediated IL- 1b secretion. PDWGF-primed PBMC were treated with SB203580 (SB), SP600125 (SP), UO125 (UO), TPCK, or PDWGF, alone or in combination for 24 h. (A) IL-1b was quantified in cell supernatants by ELISA. Mean 6 SD, n = 5 independent experiments. ***P,0.001 vs. PDWGF-treated cells. (B) Pro-IL-1b levels were examined in cell lysates by immunoblot- ting from 5 experiments. b-actin was used as a loading control. doi:10.1371/journal.pone.0062426.g004

Article Snippet: The concentrations of human IL-1b, IL-1a, IL-18 and TNF-a as well as murine IL-1b and TNF-a were measured by commercial ELISA Duo Set Kits (R&D Systems) or ELISA MAX kits (Biolegend) according to manufacturer instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Control